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CELLnTEC Advanced Cell Systems AG
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Image Search Results
Journal: Frontiers in Immunology
Article Title: A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation
doi: 10.3389/fimmu.2023.1276151
Figure Lengend Snippet: Engineering of human 3D immune competent full-thickness skin models. (A) Primary human foreskin keratinocytes are seeded onto feeder cells and harvested after six days of cultivation and seeded together with THP-1-derived iDCs (ratio 1:2) onto dermis models based on a solid collagen matrix and primary fibroblasts. After 48 h of cultivation in a submerse phase, the skin models are lifted into an air-liquid interphase. After 10 days, the skin models are cryo-sectioned for histological analysis or treated with sensitizers for 24 h and enzymatically dissociated. Created with BioRender.com . (B) The full-thickness skin model is characterized by a diameter of 1.4 cm and a height of 0.3 cm. The photo was taken on ALI d10 and depicts our immune competent skin model with a fully differentiated epidermis.
Article Snippet: 5 x 10 5 Keratinocytes in P2 were seeded together with 1 x 10 6 THP-1-derived iDCs (ratio 1:2) in 1 mL keratinocyte medium (Phenion, #K CM-250) onto dermis models based on a solid and porous collagen matrix ( , ) and
Techniques: Derivative Assay